Journal: International journal of biological macromolecules
Article Title: Aberrant serum-derived FN1 variants bind to integrin β1 on glomerular endothelial cells contributing to thin basement membrane nephropathy.
doi: 10.1016/j.ijbiomac.2024.136282
Figure Lengend Snippet: Fig. 5. Expression changes of key GBM proteins and their response to FN1 Variants in TBMN. Immunofluorescence experiments assessed the expression of Laminin α5β2, COL4A3/4/5 and Integrin β1 in the GBM. The findings revealed non-homogeneous linear structure expression of these key GBM proteins in the presence of FN1 variants, indicating their potential involvement in the pathogenesis of TBMN. HC = healthy control.
Article Snippet: The antibodies used were list below: anti-FN1 antibody (1:50, ab2413, Abcam), anti-Fibronectin-IST9 antibody (1:50, sc-59,826, Santa Cruz), anti-Human CD31 antibody (1:50, AF806, R&D Systems), antiIntegrin β1 antibody (1:100, #34971S, CST), anti-COL4A3 antibody (1:50, #7076, Chondrex), anti-COL4A4 antibody (1:50, #7073, Chondrex), anti-COL4A5 antibody (1:50, #7077, Chondrex), anti-Laminin α5 antibody (1:200, ab210957, Abcam), anti-Laminin β2 antibody (1:200, Abcam), anti-HA-Tag antibody (1:200, T0008, Affinity), anti-Integrin β1 antibody (1:50, A21234, Abclonal), anti-Laminin α5 antibody (1:50, MAB-1924, Millipore), anti-Laminin α5 antibody (1:50, NBP1–18714, Novus Biologicals), anti-Laminin β2 antibody (1:50, sc-59,980, Santa Cruz), anti-Laminin γ1 antibody (1:50, sc-17,751, Santa Cruz), donkey polyclonal secondary antibody to rabbit Alexa FluorTM 555 (1:400, A31572, Thermo Scientific), donkey polyclonal secondary antibody to sheep Alexa Fluor® 647 (1:400, ab150179, Abcam), goat polyclonal secondary antibody to mouse Alexa FluorTM 488 (1:400, A-11001, Thermo Scientific), goat polyclonal secondary antibody to rabbit Alexa Fluor® 555 (1:400, ab150078, Abcam), goat polyclonal secondary antibody to rat Alexa Fluor® 647 (1:400, ab150159, Abcam), DAPI (1:1000, C1002, Beyotime Biotechnology).
Techniques: Expressing, Immunofluorescence, Control
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